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Product description: DNase I (RNase free) is an endonuclease that digests single- and double-stranded DNA. It nonspecifically cleaves DNA to release di-,tri- and oligonucleotide products with 5'-phosphorylated and 3'-hydroxylated end.
Source: Recombinant E.coli
Concentration and Size: 1U/μL
Unit Definition: One unit is defined as the amount of enzyme required to completely degrade 1μg of λDNA in 10 minutes at 37℃.
Storage Buffer: 50mM Tris-HCl (pH7.5, 25℃), 10mM CaCl2 and 50% Glyercol
Companion Product: 10X DNase I Reaction Buffer, Cat#ON-077; 100mM Tris-HCl pH7.6, 25℃, 25mM MgCl2, 5mM CaCl2
DNase I treatment after IVT reaction:
1. Directly add DNase I (1unit DNase I per ug of DNA template) to IVT Reaction, mix well and incubate 30 min at 37°C.
2. Add 0.5M EDTA to a final concertation over one millimole than divalent cations.
3. Heat inactivate at 75℃ for 10min.
Storage Conditions: -20℃
Quality Assurance: Free of RNase activities.
Physical Purity: ＞95% by SDS-PAGE.
1. A F Worrall，B A Connolly, J，Biol，Chem. 265,21889–21896 (1990).
Ching-Ying Chen，Shao-Chun Lu，et al. Gene. 206, 181-184(1998).