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Product description: RNase III，E.coli is a homodimeric, divalent metal ion dependent nuclease. RNase III cleaves long double-stranded RNA(ds RNA) into short 12-30 base dsRNAs containing a 5'-PO4, 3'-OH, and a dinucleotide 3' overhang.
Source: Recombinant E.coli
Concentration and Size: 0.5U/μL
Unit Definition: One unit is the amount of enzyme required to digest 1 ug of dsRNA to siRNA in 20 minutes at 37℃ in a total reaction volume of 50 μL.
Storage Buffer: 500mM NaCl, 20mM Tris-HCl (pH 8.0, 25℃), 0.5mM EDTA, 0.5mM DTT, 50% Glyercol.
10X RNaseIII Reaction Buffer, Cat#ON-078, 500mM Tris-HCl (pH7.5, 25℃), 500mM NaCl, 10mM DTT
10X MnCl2, Cat#ON-079, 200mM MnCl2
10X EDTA, Cat#ON-080, 500mM EDTA
1. In ice, combine the following, in order:
|10X RNaseIII Reaction Buffer||10μL|
|RNase III||10-20 units|
2. Mix and incubate for 20 minutes at 37℃.
3. Add 10μL 10X EDTA to stop the reaction.
Long term (Infrequent use): -70℃
Daily/Weekly use: -20℃*
Do not store in a frost-free freezer.
Always avoid freeze-thaw cycles or exposure to frequent temperature changes. These fluctuations can greatly alter product stability.
* RNase III stores at -20℃ may result in the formation of haze. If this happens, keep the tube at room temperature until tube temperature is up to 4~10℃. Then mix gently to clarify the solution. Haze should disappear. And there is negligible loss of activity.
Quality Assurance: Free of Endonuclease, Exonuclease, and Nickase activities. Pass functional testing
Physical Purity: ＞90% by SDS-PAGE.
1. Hugh D. Robertson, Robent E. Webster, and Norton D. Zinder. The Journal of Biological Chemistry. 243, 82-91(1968).
2. Neelam Srivastava and Rai Ajit K Srivastava. Biochemistry and Molecular Biology International. 39, 171-180(1996).