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RNase III, E.coli

  • Cat.No.ON-024
  • SourceRecombinant E.coli
  • Purity>90% by SDS-PAGE
  • Storage-70℃
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Product description: RNase III,E.coli is a homodimeric, divalent metal ion dependent nuclease. RNase III cleaves long double-stranded RNA(ds RNA) into short 12-30 base dsRNAs containing a 5'-PO4, 3'-OH, and a dinucleotide 3' overhang.

Source: Recombinant E.coli

Concentration and Size: 0.5U/μL

Unit Definition: One unit is the amount of enzyme required to digest 1 ug of dsRNA to siRNA in 20 minutes at 37℃ in a total reaction volume of 50 μL.

Storage Buffer: 500mM NaCl, 20mM Tris-HCl (pH 8.0, 25℃), 0.5mM EDTA, 0.5mM DTT, 50% Glyercol.

Companion Product:

10X RNaseIII Reaction Buffer, Cat#ON-078, 500mM Tris-HCl (pH7.5, 25℃), 500mM NaCl, 10mM DTT

10X MnCl2, Cat#ON-079, 200mM MnCl2

10X EDTA, Cat#ON-080, 500mM EDTA

Related Products:

T7 RNA polymerase, Cat#ON-004

Ribonuclease Inhibitor, Human Placenta, Cat#ON-039

10X RNaseIII Reaction Buffer, Cat#ON-078

10X MnCl2, Cat#ON-079

10X EDTA, Cat#ON-080

Recommended protocol:

1. In ice, combine the following, in order:

ddH2OX μL
10X RNaseIII Reaction Buffer10μL
dsRNAX μL(10μg)
RNase III10-20 units
10X MnCl210μL
Total Volume100μL

2. Mix and incubate for 20 minutes at 37℃.

3. Add 10μL 10X EDTA to stop the reaction.

Storage Conditions:

Long term (Infrequent use): -70℃

Daily/Weekly use: -20℃*

Do not store in a frost-free freezer.

Always avoid freeze-thaw cycles or exposure to frequent temperature changes. These fluctuations can greatly alter product stability.

* RNase III stores at -20℃ may result in the formation of haze. If this happens, keep the tube at room temperature until tube temperature is up to 4~10℃. Then mix gently to clarify the solution. Haze should disappear. And there is negligible loss of activity.

Quality Assurance: Free of Endonuclease, Exonuclease, and Nickase activities. Pass functional testing

Physical Purity: >90% by SDS-PAGE.

1. Hugh D. Robertson, Robent E. Webster, and Norton D. Zinder. The Journal of Biological Chemistry. 243, 82-91(1968).

2. Neelam Srivastava and Rai Ajit K Srivastava. Biochemistry and Molecular Biology International. 39, 171-180(1996).

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