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Taq DNA polymerase

  • Cat.No.ON-007
  • SourceRecombinant E.coli
  • Purity>95% by SDS-PAGE
  • Storage-20℃
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Product description: Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5'→ 3' polymerase activity and 5'→ 3' exonuclease activity. The PCR product can be cloned directly into a T-vector(TA cloning) due to the addition of an adenosine(A) to the 3' end. The thermostable enzyme is a single polypeptide chain with a molecular weight of approximately 94kD.

Source: Recombinant E.coli

Concentration and Size: 5U/μL

Unit Definition: One unit is defined as the amount of enzyme that incorporates 10nmols of total dNTPs into acid-insoluble DNA in 30min at 74℃.

Storage Buffer: 20mM Tris-HCl (pH7.9, 25℃), 0.1mM EDTA, 100mM KCl, 0.5% Tween20, 0.5% NP40, 1mM DTT, 50%(v/v) Glycerol.

Companion Products: 10X Reaction Buffer, Cat#ON-012, 100mM Tris-HCl, 500mM KCl, pH9.0, 25℃; 100mM MgCl2, Cat#ON-013.

Related Products:

pfu DNA polymerase, Cat#ON-051

Uracil-DNA Glycosylase (UNG), heat-labile, Cat#ON-054

Uracil-DNA Glycosylase(UNG), E.coli, Cat#ON-056

10X Reaction Buffer for Taq, Cat#ON-012

25mM MgCl2, Cat#ON-060

100mM MgCl2, Cat#ON-013

1. Recommended reaction mixture:

Final VolumeFinal conc
10X Reaction buffer5μL1X
dNTP Mix, 10mM each10.2mM each
25mM  MgCl231.5mM
upstream primerX μL0.1-1.0μM
downstream primerY μL0.1-1.0μM
Taq (5U/μl)0.25μL1.25U
Template DNAZ μL<0.5μg/50μL
Nuclease-Free H2Oto 50μL

2. Recommended reaction conditions:

CycleTimeTemp ℃
Initial Denaturation12min95
Final extension15min72

Optimal enzyme concentration: 0.5U-5U per 50μL volume (50μL volume: 1.25U recommended)

Optimal MgClconcentration: 1.5mM (1~5mM)

Storage Conditions: -20℃

Quality Assurance: Free of Endonuclease, Exonuclease, Nickase and RNase activities.

Free of 16s rDNA.

Physical Purity: >95% by SDS-PAGE.

1. Lawyer FC,et al. PCR Methods Appl. 2(4):275-87(1993).

2. Chien A, Edgar DB, Trela JM.J. Bacteriol 127(3):1550-7(1976).

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