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Product description: BspQI is a thermostable Type IIS restriction endonuclease (REase) with the recognition sequence 5'-GCTCTTCN1/N4-3'
Source: Recombinant E.coli
Concentration and Size: 10U/μL
Unit Definition: One unit is defined as the amount of enzyme required to digest 1μg of λDNA in 1X Cut Buffer following 1 hour incubation at 50°C in a total reaction volume of 50μL.
Storage Buffer: 20mM Tris-HCl, 0.1mM EDTA, 500mM KCl, 1mM DTT, 0.1%Triton X-100, 50%(v/v) glycerol, pH7.0, 25℃.
Companion Product: 10X Cut Buffer with BSA, Cat#ON-187, 500mM Tris-HCl, 1000mM NaCl, 100mM MgCl2, 1mg/mL BSA, pH7.9, 25℃.
Restriction Digest Protocol:
1. On ice, combine the following reaction components in the order given,
|5μL||10X Cut buffer|
|50μL||Total reaction volume|
2. Incubate at 50℃, 60min
3. Heat inactivity enzyme by incubating at 80℃, 2min
Methylation Sensitivity: Not CpG methylation-sensitive,?Not dam methylation-sensitive,?Not dcm methylation-sensitive.
Enzyme stability: BspQI half-life?period at 50℃ is 2 hours in 1X Cut buffer.
Storage Conditions: -20℃
Quality Assurance: Free of endonuclease, exonuclease and RNase activities.
Physical Purity: ＞95% by SDS-PAGE.