Applications of nucleotides in molecular diagnostics:


Polymerase Chain Reaction (PCR): PCR is a fundamental technique that amplifies specific DNA regions. It utilizes nucleotides as substrates for DNA polymerase enzymes, which synthesize complementary DNA strands to the target sequence. By repeatedly cycling through heating, annealing, and extension steps, PCR exponentially increases the amount of target DNA, enabling its detection and analysis. 


Nucleic Acid Hybridization: This technique relies on the specific base pairing between complementary nucleotide sequences. Single-stranded DNA probes, containing nucleotides complementary to the target sequence, are used to identify and bind to specific DNA or RNA molecules in a sample. The probes can be labelled with fluorescent dyes or other detectable markers, allowing visualization and quantification of the target molecules.


Sanger Sequencing: This traditional DNA sequencing method utilizes dideoxynucleotides (ddNTPs), modified nucleotides that lack a 3'-hydroxyl group essential for further phosphodiester bond formation. By incorporating ddNTPs during DNA synthesis, the chain extension terminates at specific points, revealing the sequence of the DNA strand.


Next-Generation Sequencing (NGS): NGS technologies employ sequencing-by-synthesis approaches. Nucleotides labelled with fluorescent dyes are sequentially incorporated into growing DNA strands. The detection of each incorporated nucleotide allows for the identification of the corresponding base in the target sequence. NGS offers high-throughput sequencing of entire genomes or targeted regions, aiding in genetic analysis, variant detection, and personalized medicine.


In Situ Hybridization (ISH): This technique involves hybridizing probes directly to cells or tissues fixed on slides. By employing nucleotide probes specific for a particular gene or mRNA, ISH enables the visualization of gene expression patterns within cells or tissues.



0 product(s) in total


ATP 100mM solution

Catalog No.: R1331
Molecular Formula: C10H13N5Na3O13P3
CAS No.: 987-65-5

GTP 100mM solution

Catalog No.: R2331
Molecular Formula: C10H13N5Na3O14P3
CAS No.: 36051-31-7

CTP 100mM solution

Catalog No.: R3331
Molecular Formula: C9H13N3Na3O14P3
CAS No.: 123334-07-6

UTP 100mM solution

Catalog No.: R5331
Molecular Formula: C9H12N2Na3O15P3
CAS No.: 19817-92-6

ITP 100mM solution

Catalog No.: R6331
Molecular Formula: C10H12N4Na3O14P3
CAS No.: 35908-31-7

NTP 25mM Mix

Catalog No.: RN31

dATP, 100mM solution

Catalog No.: D1331
Molecular Formula: C10H13N5Na3O12P3
CAS No.: 1927-31-7

dGTP, 100mM solution

Catalog No.: D2331
Molecular Formula: C10H13N5Na3O13P3
CAS No.: 93919-41-6

dCTP, 100mM solution

Catalog No.: D3331
Molecular Formula: C9H13N3Na3O13P3
CAS No.: 102783-51-7

dTTP, 100mM solution

Catalog No.: D4331
Molecular Formula: C10H14N2Na3O14P3
CAS No.: 18423-43-3

dUTP, 100mM solution

Catalog No.: D5331
Molecular Formula: C9H12N2Na3O14P3
CAS No.: 102814-08-4

dITP 100mM

Catalog No.: D6331
Molecular Formula: C10H12N4Na3O13P3
CAS No.: 95648-77-4

dNTP 2.5mM Mix

Catalog No.: DN34

dNTP 10mM Mix

Catalog No.: DN31

dNTP 25mM Mix

Catalog No.: DN32

N1-Me-pUTP, 100mM solution

Catalog No.: R5-027
Molecular Formula: C10H14N2Na3O15P3

2'-O-Me-CTP, 100mM Lithium Salt Solution

Catalog No.: R3-017
Molecular Formula: C10H15Li3N3O14P3

2'-O-Me-GTP, 100mM Lithium Salt Solution

Catalog No.: R2-011
Molecular Formula: C11H15Li3N5O14P3

2'-O-Me-UTP, 100mM Lithium Salt Solution

Catalog No.: R5-016
Molecular Formula: C10H14Li3N2O15P3

pUTP, 100mM solution

Catalog No.: R5-022
Molecular Formula: C9H12N2Na3O15P3
CAS No.: 1175-34-4 (free acid)